Figure 1.

Inhibit miRNA in Caenorhabditis elegans using a dextran-conjugate of antisense 2'-O-methyl oligoribonucleotide. (a) Chemical synthesis of dextran-(^as-2'OMelin-4)₈, or D-(^as-2'OMelin-4)₈, a dextran conjugate of an antisense reagent against lin-4. (b) Dose response of D-(^as-2'OMelin-4)₈ in causing egg laying defectives (Egl) via lin-4 inhibition. Controls include ^as-2'OMelin-4, a lin-4 antisense 2'-O-methyl oligoribonucleotide without dextran; D-(^s-2'OMelin-4)₈ and D-(^as-2'OMemiR-237)₈, dextran conjugates containing either lin-4 sense or miR-237 antisense 2'-O-methyl oligoribonucleotide. Error bars are standard errors of two independent injections. Each time ~ eight worms were injected and 50 or more labelled embryos from injected worms were scored for Egl when they reached adults. (c, d) Example images of a normal worm (c) and an Egl worm (d) labelled with dextran-(^as-2'OMelin-4)₈.