Additional file 1:

Figure S1. (A) Gene structure of FBW2 showing the location of primers used in real time polymerase chain reaction and the abundance of FBW2 messenger RNA in sqn-1 and mutants doubly mutant for sqn-1 and different alleles of fbw2. All target genes were normalized to EIF4. (B) Alignments of the Arabidopsis F-box genes most closely related to At4g08980 (FBW2): At4g05497 (FBW9), At4g05460 (FBL20), At5g57900 (SKIP1). (C) Alignments of the amino acid sequences of FBW2 and its predicted orthologs in other flowering plants. All alignments were made using the following assembled contigs from PlantGDB unless otherwise noted: Citrus sinensis (PUT-157a-Citrus_sinensis-6728477), Gossypium raimondii (PUT-157a-Gossypium_raimondii-11427), Glycine max (PUT-161a-Glycine_max-76896), Oryza sativa Japonica (Genbank AK070008), Asparagus officinalis (Genbank CV288647), Vitis vinifera (PUT-157a-Vitis_vinifera-16230), Nicotiana tabacum (PUT-163a-Nicotiana_tabacum-55992858), Lactuca sativa (PUT-157a-Lactuca_sativa-30499), Pinus taeda (PUT-157a-Pinus_taeda-79282550).

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Additional file 2:

Figure S2. (A) Western blot comparing levels of ARGONAUTE1 (AGO1) and yellow fluorescent protein-AGO10 in plants with and without FBW2ox constructs. Anti-AGO1 and anti-GFP antibodies were used to probe each blot. Ponceau staining was used as a loading control. (B) Levels of AGO1-FLAG do not decrease in plants treated with the protease inhibitor MG132. Blots were probed with anti-FLAG monoclonal antibody or anti-ubiquitin antibody. The anti-ubiquitin antibody demonstrates an overall increase in ubiquitination in MG132 treated plants, as expected from a decrease in proteasome activity.

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Additional file 3:

Table S1. Polymerase chain reaction primers used in this study.

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