Figure 5.

Role of miR-372 and miR-373 in Helicobacter pylori-induced cell cycle arrest in AGS cells. (A) DNA synthesis rate of uninfected AGS cells or cells infected for 24 h with wild-type H. pylori or its isogenic mutants ΔCagA and ΔCagE. (B,C) DNA synthesis rate of parental AGS cells (mock), or treated cells infected with or without wild-type H. pylori for 24 h. Previously, cells were transfected either with 100 nM of a mixture (1:1) of anti-miR-372 and miR-373 antisense oligonucleotides (as372-373), 25 nM miR-372 and miR-373 double-stranded RNA mimics or 100 nM scrambled oligonucleotides (sc372-373) (B), or treated with 20 nM of anti- large tumor suppressor homolog 2 (LATS2) siRNA or control siRNA (C). DNA synthesis level was measured by 5-bromo-2'-deoxyuridine (BrdU) pulse labeling. Bars represent the relative BrdU incorporation level compared to that of uninfected (A) or uninfected parental (B,C) AGS cells. Data are mean ± SD of two independent experiments. *P < 0.05.