Table 1 |
|
|
Structural statistics for the 10 lowest energy-minimized conformers of Drosha-double-stranded RNA-binding domain (dsRBD) |
|
|
Statistic |
Value |
|
|
|
|
NOE distance restraints: |
|
|
Intraresidue |
98 |
|
Sequential |
111 |
|
Medium range (i, i + 2 to 4) |
74 |
|
Long range (i, i>4) |
206 |
|
Total |
489 |
|
Dihedral restraints: |
189 |
|
Hydrogen bond (HBDA): |
27 |
|
Ensemble RMSD: |
|
|
Backbone secondary structure |
0.44 |
|
Heavy atoms secondary structure |
0.86 |
|
Violations: |
|
|
NOE |
0 |
|
Dihedral |
0 |
|
HBDA |
1.4 |
|
RMS experimental: |
|
|
NOE |
0.02 ± 0.005 |
|
Dihedral |
0.415 ± 0.077 |
|
HBDA |
0.006 ± 0.006 |
|
RMS covalent geometry: |
|
|
Bonds |
0.003 ± 0.000 |
|
Angles |
0.514 ± 0.009 |
|
Impropers |
0.362 ± 0.013 |
|
Ramachandran space: |
|
|
Most favored region |
81.6% |
|
Additionally allowed |
17.0% |
|
Generously allowed |
1.4% |
|
Disallowed |
0 |
|
|
|
|
HBDA = hydrogen bond distance angle; NOE = nuclear Overhauser effect; RMSD = root mean square deviation. |
|
|
Mueller et al. Silence 2010 1:2 doi:10.1186/1758-907X-1-2 |
|
